![]() No conjugate is needed for fluorescent blot detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Packaging Options Number of Applications (Volume) Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range.Molecular weight sizing of proteins after blot development.Monitoring blot transfer efficiency with easy orientation via multicolor standards.These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Suppose I calculate through formula N1V1N2V2. Burnette in 1981 after the eponymous Southern blot for DNA and the consequent coinage of the northern blot in 1977 for RNA.12 Western blotting separates, detects, and identifies one or more proteins in a complex mixture. I want to load 50 ug/20 ul/well of SDS-PAGE for Western blot and I have protein concentrations 6.18, 4.9, 5.76, 6.53, 4.43, 5.83, 5.11& 7.46 ug/ul. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. The name ‘western’ blot was first coined by Dr. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection.
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